TABLE 1.

Bovine Somatotropin (GH) Induces Members of IGF1 Ternary Complex

Time (h)	IGFALS mRNA	IGF1 mRNA	IGFBP3 mRNA
0	1.0 ± 0.1	1.0 ± 0.4	1.0 ± 0.1
2	1.3 ± 0.3	1.5 ± 0.2	2.1 ± 0.5
3	1.8 ± 0.3	1.7 ± 0.5	1.3 ± 0.5
4	1.9 ± 0.3	2.3 ± 0.4	2.9 ± 0.6*

Note. Mice were treated with GH once and sacrificed 2, 3, and 4 h later. Naïve mice (0 h) were sacrificed together with treated mice. Liver sections were collected and analyzed for IGFALS, IGF and IGFBP3 mRNA expression by real-time PCR. Data are mean ± SEM (n = 5) of fold changes in mRNA relative to an untreated group (onefold). Mean with asterisk differ from untreated group (P < 0.05).