Fig. 2.

(a) Representative Srpx-based assays for Xi. Mouse kidneys from the indicated strains were analysed by multiplex RT-PCR and subsequent digestion as described in Animals and Methods and Table 1. Sau96I digests the B6, but not the 129 Srpx RT-PCR product, generating fragments of 154 and 221 bp from the original 375-bp product. (b) Distribution by group of Xi, measured as allele-specific Srpx expression. (c) Correlation between Xi and 6-month urine protein-to-creatinine ratio. Group 1 mice are indicated by filled grey circles and Group 2 by open circles. (d) Correlation between Xi and 6-month plasma urea nitrogen. Group 1 mice are indicated by filled grey circles and Group 2 by open circles.