. 2009 Oct 23;25(3):764–769. doi: 10.1093/ndt/gfp551

Table 1.

Single-nucleotide polymorphisms, gene-specific primers and expected band sizes for undigested and digested PCR products used in allele-specific mRNA expression assays

Gene	refSNP ID	PCR primer sequences	Size (bp)	Enzyme	Fragments B6 Allele	Fragments 129 Allele
Srpx	rs30730568	F:5′-TCATCGGCAGGATCAGAGCAAAG-3′	375	Sau96I	154 221	375
Srpx	rs30730568	R:5′-GCAAAGAAAGCACATAATAAAATAGAC-3′	375	Sau96I	154 221	375
Rpgr	rs33492631	F:5′-AGTACAATGAAAATCCAAAAGGACAC-3′	602	BsaAI	602	95 507
Rpgr	rs33492631	R:5′-GCTTCATTCGTCTTTCAAATATAAAAATAG-3′	602	BsaAI	602	95 507
Aff2	rs13483812	F:5′-AAACAGATTCATCTACATCTGACTCC-3′	441	BsrDI	166 275	441
Aff2	rs13483812	R:5′-CAGGGATTTTCTCTGCAGTTTCTG-3′	441	BsrDI	166 275	441