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. Author manuscript; available in PMC: 2010 Jul 13.
Published in final edited form as: Biochemistry. 2000 Dec 26;39(51):15791–15798. doi: 10.1021/bi001453j

FIGURE 4.

FIGURE 4

Subunit exchange rate by FET analysis. AlexaFluor350-conjugated WTαA and αA R116C (fluorescence donors) were incubated at 37 °C in PBS with either nonconjugated WTαA or AlexaFluor430-conjugated WTαA as the fluorescence acceptor. The increase in energy transfer, as calculated using eq 5, was monitored by measuring the fluorescence of the donor in the presence and absence of acceptor and then scaled as described in Experimental Procedures. WTαA subunits exchanged into WTαA (●) and αA R116C (○) homocomplexes with initial rates of (8.7 ± 0.2) × 10−5 and (2.5 ± 0.1) × 10−5 mol exchanged per second, respectively.