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. Author manuscript; available in PMC: 2010 Jul 13.
Published in final edited form as: Biochemistry. 2000 Dec 26;39(51):15791–15798. doi: 10.1021/bi001453j

FIGURE 6.

FIGURE 6

CLA of heterocomplexes. The ability to suppress the aggregation of 2 nmol of aldose reductase (■) at 52 °C in PBS for 3:1 (WTαA:WTαB) and 1.5:1.5:1 (WTαA:αA R116C:WTαB) mixed samples was compared. α-Crystallin subunits were combined and incubated at 37 °C for 15 h prior to use. The 3:1 sample assay (●) required 0.8 nmol of complex to achieve 50% inhibition of aggregation, while the 1.5:1.5:1 sample mixture (○) required 1.2 nmol of complex. These data correspond to a 1.5-fold reduction in overall CLA. Light scattering was monitored at 390 nm.