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. Author manuscript; available in PMC: 2010 Jul 13.
Published in final edited form as: Biochemistry. 2000 Dec 26;39(51):15791–15798. doi: 10.1021/bi001453j

FIGURE 7.

FIGURE 7

Membrane binding capacity of WTαA and αA R116C. Assays were performed using 500 µg of plasma membranes and a varied amount of either 14C-WTαA or 14C-αA R116C. The amount bound was calculated from the radioactivity of the membrane pellet and the specific activity for each α-crystallin complex. The binding capacity was calculated using the asymptote of each curve; values were 7.1 ± 0.7 ng of αA/µg of membrane for WTαA(●) and 55 ± 19 ng of αA/µg of membrane for αA R116C (○).