A. LNCaP cells had less proliferation in media containing the LF group mouse serum compared to the cells grown in media containing the HF group serum from Hi-Myc mice. The cell proliferation was measured after 48 hour incubation in the media containing 10 % mouse serum. N=8 for each diet group.
B. Myc-CaP cells grown in media containing 10 % Myc-mouse serum had less proliferation with LF group serum compared to the serum from HF diet group after 24 hour incubation. N= 5 for each group
All experiments were performed in triplicate with the serum from individual mice (not pooled). Data is expressed as a percentage of the cell growth in media containing 10 % FBS. Values are means ± SEM. *P<0.05. Proliferation was determined by measuring colorimetric changes of MTS tetrazolium compound into colored formazan product by NADPH or NADH produced by active cells.