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. Author manuscript; available in PMC: 2010 Jul 13.
Published in final edited form as: J Biol Chem. 2005 Aug 22;280(45):37782–37789. doi: 10.1074/jbc.M505278200

FIGURE 4. Transient overexpression of PAPP-A increases myoblast proliferation and differentiation.

FIGURE 4

C2C12 myoblasts were transfected with 2 µg/well of either pFLAG empty vector DNA or PAPP-A/pFLAG plasmid DNA. The production of recombinant PAPP-A after transfection in each experiment was measured in culture supernatants and quantified using purified protein (A and C). After 16 h of transfection, the medium was replaced with either DMEM containing 5% fetal calf serum (for proliferation assay) or 2% horse serum (for differentiation study). The proliferation of myoblasts was studied using AlamarBlue dye, whereas the differentiation was monitored by assaying the creatine kinase (CK) activity. The data presented here show an increase in the proliferation (*, p <0.05 versus vector alone) (B) and the differentiation (#, p <0.05 versus vector alone) (D) in PAPP-A-transfected C2C12 cultures as compared with C2C12 cultures transfected with vector alone.