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. Author manuscript; available in PMC: 2010 Jul 13.
Published in final edited form as: J Biol Chem. 2005 Aug 22;280(45):37782–37789. doi: 10.1074/jbc.M505278200

FIGURE 5. Effect of overexpression of mutant PAPP-A on the proliferation of C2C12 myoblasts.

FIGURE 5

C2C12 myoblasts were transfected with either full-length (amino acids 1–1547) or truncated (amino acids 1–920) PAPP-A plasmid as described in Fig. 4A. Empty vector DNA was used to adjust the total amount of DNA to 2 µg/well. 40 µl of CM was treated without (A) or with (B) reducing agent β-mercaptoethanol and subjected to immunoblot analysis with FLAG antibody. C, proliferation of transfected C2C12 myoblasts was determined in 5% fetal calf serum medium using AlamarBlue assay as described under “Experimental Procedures.” The data presented here show that overexpression of full-length PAPP-A, but not truncated PAPP-A, significantly increased the proliferation of C2C12 myoblasts (*, p <0.05 versus vector alone).