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. Author manuscript; available in PMC: 2010 Jul 13.
Published in final edited form as: Biochemistry. 2006 Oct 3;45(39):11737–11743. doi: 10.1021/bi060741y

FIGURE 3.

FIGURE 3

Sequence of assembly events. Positions 283 and 291 in the vimentin L2 domain display contacts earlier in the assembly from urea. As shown in the inset, dialysis to lower concentrations of urea generates broadened spectra. The broadening reflects restricted side chain and backbone motions that accompany folding and, in the cases of sites close to each other, additional broadening due to magnetic dipolar interactions. The spectral broadening as approximated by the central (mI = 0) EPR line height is plotted as a function of urea concentration.