Fig. 3.

Analysis of liver injury after hepatic ischemia with various periods of reperfusion. (A) Liver injury as assessed by alanine aminotrans-ferase (ALT) levels was significantly decreased in mice null for CD39, when compared to wild-type mice after 3 and 24 hours of reperfusion. (B) Cytokine profiling using arrays after 3 hours of reperfusion revealed decreased levels of circulating cytokines with the exception being IL-10. Representative liver sections of serial hematoxylin & eosin staining after 4 days of reperfusion; (C) wildtype and (D) CD39-null. (E) Results of morphometric analysis of area of necrosis revealed significantly increased area of necrosisin wild-type mice compared to mice null for CD39. Values are means ± standard deviation of at least four animals per time point. Levels of significance were assessed by unpaired t tests. P values are as indicated; asterisks indicate P < 0.05 in (B).