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. Author manuscript; available in PMC: 2010 Jul 13.
Published in final edited form as: Sci Transl Med. 2010 Apr 21;2(28):28ra29. doi: 10.1126/scitranslmed.3000522

Fig. 6.

Fig. 6

Abcc8-AS reduces secondary hemorrhage and capillary fragmentation. (A) Fluorescence images of the penumbra 24 hours after hemicord C7 SCI in a rat administered CY3-Abcc8-AS (red) by constant infusion for 24 hours after SCI, showing uptake of CY3-Abcc8-AS by capillary endothelial cells at different magnifications, with intranuclear localization suggested by pink nuclei (inset). Rats were perfused to remove intravascular contents. Nuclei are labeled with DAPI (blue). Scale bar, 50 μm. (B) Penumbral tissues immunolabeled for SUR1 (red) and double-labeled for von Willebrand factor (green) 24 hours after SCI in rats given Abcc8-Scr or Abcc8-AS, showing reduced expression of SUR1 in capillaries with Abcc8-AS. Scale bar, 50 μm. Images shown are representative of findings in three rats in each group. (C) Immunoblots for SUR1 in tissues obtained 24 hours after SCI from rats administered Abcc8-Scr or Abcc8-AS. Bar graph, densitometric analysis of immunoblots (three rats per group). **P < 0.01. (D) Spinal cord sections and homogenates from rats administered Abcc8-Scr or Abcc8-AS. Bar graph, spectrophotometric quantification of extravasated blood 24 hours after SCI in spinal cord homogenates from rats administered Abcc8-Scr or Abcc8-AS after SCI (five rats per group). **P < 0.01. (E) Sections of penumbra at two magnifications immunolabeled for laminin showing fragmentation of capillaries in rats administered Abcc8-Scr and elongated, intact capillaries in rats administered Abcc8-AS. Asterisks indicate site of impact. The images shown are representative of findings in three rats per group. Bar graph, length of capillaries measured with laminin in three groups of rats, as indicated. Remote sections were from 7 mm proximal to the injury. *P < 0.05.