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. Author manuscript; available in PMC: 2011 Apr 9.
Published in final edited form as: J Mol Biol. 2010 Feb 6;397(4):1106–1118. doi: 10.1016/j.jmb.2010.01.070

Figure 4. Thermal denaturation and refolding of Aa1 VHH.

Figure 4

(A) Far UV CD spectra of Aa1 VHH obtained at 10°C (◆) before melting, 90°C (▼) after melting, and 10°C (▲) following the melting and refolding of the protein. (B) (C) Thermal denaturation (○) and refolding (□) data of Aa1 VHH obtained by CD spectroscopy at a wavelength of 216 nm (panel B) and 224 nm (panel C).