Table 1.
Properties of BoNT/A Lc binding VHH antibodies.
| VHH clone name | Sequences of CDR3 | Yeast-displayed VHH KD for BoNT/A Lc (nM) |
|---|---|---|
| A26 | EVSSGQPAVTTFWEDMYDY | 8.7 |
| A3 | YRRRHRCSAFGIANEYDY | 6.63 |
| A16 | DDPLVGRGWDGAEGYDY | 4.04 |
| Aa1a,b | DEDVTPRVMGVIPHADH | 0.03 |
| A23a,b | DEDVTPRGMGVIPYAEY | 16.94 |
| A10 c | DDGEYVIPSDQNEYEF | 76.34 |
| Aa12 c | DDGEYVIPSDQNEYEF | 30.27 |
| Aa6 a | SSDYRWSRQPFEFEN | 0.52 |
| Aa9 a | DFDTPWGASGRYDY | 4.08 |
| A8 a | DEDLLPSFVSDFDY | 229.9 |
| A21 | DLGSVGPGAEYDY | 60.74 |
| A19 a | DSYVDYEDDRLK | 4.65 |
| Aa8 a | HWDYGLGPE | 112.09 |
| Aa5 a | VSTDWTTDY | 207 |
| Aa11 | WSLEEQY | 76.08 |
a
VHH which inhibit the catalytic activity of BoNT/A Lc.
b
VHH Aa1 and A23 have the same CDR1 sequence and highly related CDR3 sequence.
c
VHH A10 and Aa12 have the same CDR3 sequence but different CDR1 and CDR2 sequences.