Decreased SCOT (a) and PC (b) proteins in islets of donors with type 2 diabetes. SCOT and PC in islets of diabetic donors were compared with those in islets of non-diabetic human controls and other cell types as controls. a Immunoblot of SCOT. Protein was quantified as 10, 15, 6, 10, 15, 15 and 10 μg protein/lane (lanes 1 to 7, from left to right). b Streptavidin blot. Protein was quantified as 15 μg protein/lane in lanes 1 to 6 and 5 μg protein/lane in lanes 7 and 8 (from left to right). The actin band shows equal loading of protein across the lanes. T2D, type 2 diabetes. Cntrl, control; mito, mitochondrial; hum, human