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. 2010 Jun 24;4:20. doi: 10.3389/fnana.2010.00020

Figure 14.

Figure 14

Correlative light and electron microscopy of PHF-tau-ir neurons in the pyramidal layer of CA1 from patient P1. (A) Photomicrograph of a plastic-embedded 100 μm-thick section immunostained for PHF-tau. (B) Photomicrograph of a 2-μm-thick semithin section stained with 1% toluidine blue obtained from the section shown in (A). (C), (F), higher magnification of cells 1 (pattern I of PHF-tau-immunostaining) and 7 (pattern II of PHF-tau-immunostaining), respectively, also shown in (A) and (B). (D), (E,G), electron micrographs of neurons 1 and 7, respectively, obtained after the resectioning of the semithin section shown in (B). The cytoplasm of neuron 1 (D) has a normal appearance, whereas the cytoplasm of neuron 7 (G) and (E) has NFT of paired helical filaments stained for PHF-tau (asterisks in E and I). (H,I,J) Electron micrographs illustrating the neuropil around PHF-tau-ir cells. Arrows indicate some normal looking synaptic contacts situated in close proximity of the PHF-tau-ir cell bodies which have been pseudo-colored in blue. Scale bar: (A), 104 μm; (B), 95 μm; (C,F), 23 μm; (D), 1.5 μm; (E), 1.1 μm; (G), 10 μm; (H), 0.6 μm; (I), 0.8 μm; (J), 0.5 μm.