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. 2010 Jun 24;4:20. doi: 10.3389/fnana.2010.00020

Figure 3.

Figure 3

Confocal microscopy of sections double stained with an anti-PHF-tau antibody (red) and Thioflavine-S (TS, green), which labels PHF-tau forming NFT. (A,B), (D,E), (G,H), and (J,K) pairs of confocal images from the same section and field in the CA1 hippocampal region of AD patient P1, P7, P4, and P3, respectively. Panels (C,F,I,L) were obtained by combining these images (A) and (B), (D) and (E), (G) and (H), (J) and (K), respectively. Note that type I PHF-tau-ir neurons (one asterisk) were free of NFT, whereas type II neurons (two asterisks) contained NFT (arrows). Scale bar: (A–C), 21 μm; (D–F), 24 μm; (G–L), 31.5 μm.