. Author manuscript; available in PMC: 2011 Jul 14.

Published in final edited form as: J Am Chem Soc. 2010 Jul 14;132(27):9363–9372. doi: 10.1021/ja101588r

Table 1.

Kinetics of PARP-1 and Tankyrase-1 with NAD, 6- and 8-alkyne-NAD.

PARPs	Substrates	PARP activity			NADase activity
PARPs	Substrates	k_cat (s⁻¹)	K_m (μM)	k_cat/K_m (s⁻¹M^–1)	k_cat (s⁻¹)	K_m (μM)	k_cat/K_m (s⁻¹M⁻¹)
PARP-1	NAD	5.2 ± 0.1	97 ± 7	5.4 × 10⁴	0.99 ±0.06	203 ±33	4.88 × 10³
	6-alkyne-NAD	0.73 ± 0.04	147 ± 23	5.0 × 10³	0.94 ±0.1	1891 ± 273	497
	8-alkyne-NAD^*	N/A	N/A	0.51	N/A	N/A	11.90
Tankyrase-1	NAD	0.51 ± 0.04	1125 ± 133	453	0.54 ± 0.02	607 ± 29	890
	6-alkyne-NAD^*	N/A	N/A	122	N/A	N/A	252
	8-alkyne-NAD^*	N/A	N/A	0.29	N/A	N/A	3.41

(The reaction rate of PARP-1 or Tankyrase-1 with 6- or 8-alkyne-NAD was linear with the 6- or 8-alkyne-NAD concentration used (10 μM − 1 mM). Thus k_cat and K_m could not be measured and only k_cat/K_m was obtained.)