Figure 5.

Parvalbumin-positive inhibitory boutons. (A) Projection images of deconvolved confocal image stacks (13 z-planes taken 0.22 μm apart) labeled for PV in Cryostat sections (40 μm). Arrowheads indicate a chandelier cell cartridge. An unlabeled pyramidal cell (P) is surrounded by PV+ presumptive basket cell boutons. (B,C) The entire PV cartridge was masked (B) followed by the masking of puncta that make up the cartridge using a modification of our iterative segmentation methodology (C). (D) Shows the mask objects remaining after using our masking method followed by the subtraction of mask objects that colocalized with the mask in (B). The objects thus represent the PV+ boutons not located within cartridges. Bar = 10 μm.