FIG. 2.

Expression of mCeacam1 mRNA in the liver, ileum, spleen, and brain in homozygous gene-replaced B6 (1a/1a), SJL (1b/1b), and cB61ba (1ba/1ba) mice. cDNA was synthesized from the RNA isolated from these tissues of four mice in each mouse strain by using Isogen, as described in Materials and Methods, and subjected to real-time qPCR to detect both 1a and 1b (1a + 1b), only 1a (1a-specific), or only 1b (1b-specific). Regions detected by qPCR are indicated by fine black lines in Fig. 1Ab. qPCR of GAPDH cDNA was used as an internal control. Error bars represent the standard deviations of the results from four independent samples.