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. 2010 Apr 21;84(13):6288–6296. doi: 10.1128/JVI.00274-10

FIG. 6.

FIG. 6.

XMRV wild-type virus replication in prostate cancer and immortalized prostate epithelial, smooth muscle, and fibroblast cell lines. (A) XMRV env gene expression in prostate cancer cell lines was detected by RT-PCR. (B) Expression of the viral Gag protein in prostate cancer cells was detected by Western blotting. (C) XMRV env detection in all of the prostate fibroblast cell lines by RT-PCR. Detection of viral Gag protein expression was performed by uising Western blots in fibroblast cells (Pf179T) (D) and epithelial (Ep156T) and smooth muscle (Pm151T) cells (F). Five times the amount of Pf179T protein was loaded compared to that of the DU145 cells, since XMRV is known to replicate slowly in the Pf179T cells. (E) XMRV env expression in immortalized epithelial (Ep156T) and smooth muscle (Pm151T) cell lines was detected by RT-PCR.