FIG. 5.

NSP5 colocalizes with perilipin A in VLS. MA104 cells were infected with a vaccinia virus recombinant expressing T7 polymerase and transfected after 1 h. (A) Transfection of plasmid expressing NSP2 only. (B) Transfection of plasmid expressing NSP5 only. (C) Cotransfection of NSP2- and NSP5-expressing plasmids, leading to the production of VLS (see magnification in inserts). Perilipin A only colocalizes with VLS and not the individual nonstructural rotavirus proteins. (D) Vaccinia virus-infected and mock-transfected controls. Costaining was by anti-NSP2, anti-NSP5, and anti-perilipin A antibodies, followed by staining with secondary antibodies labeled with Alexa Fluor 488 and Alexa Fluor 633. Scale bar, 10 μm.