FIG. 6.

RASCAL colocalization with lamin B but not lamin A/C in cytoplasmic vesicles. Confocal images of TB40/E-infected HF (MOI of 5) harvested at 96 hpi and stained with affinity-purified rabbit anti-RASCAL polyclonal Abs followed by Alexa Fluor 594-conjugated goat anti-rabbit Abs (red), with monoclonal anti-lamin B Abs followed by FITC-conjugated anti-mouse Abs (green) (A to F), or with monoclonal anti-lamin A/C Abs followed by FITC-conjugated anti-mouse Abs (green) (G to I). The area magnified in panels D to F is framed by a square box in panels A to C. Arrowheads indicate the points of close contact between the cytoplasmic vesicles and the nuclear lamina. Bar size, 5 μm.