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. 2010 Apr 14;84(13):6483–6496. doi: 10.1128/JVI.02462-09

FIG. 7.

FIG. 7.

Localization of UL50-HA, UL53-FLAG, and RASCALTB40/E in transfected cells. (A to D) Confocal images of HEK293T transiently transfected with expression plasmids encoding UL50-HA (A and B) or UL53-FLAG (C and D) and stained with mouse anti-HA Ab (A), mouse anti-FLAG Abs (C), or affinity-purified rabbit anti-RASCAL polyclonal Abs (B and D) followed by FITC-conjugated goat anti-mouse Abs (green) or Alexa Fluor 594-conjugated goat anti-rabbit Abs. (E to M) Confocal images of HEK293T coexpressing UL50-HA and UL53-FLAG (E to F), UL53-FLAG and RASCALTB40/E (H to J), or UL50-HA and RASCALTB40/E (K to M). Cells were stained with rabbit anti-HA and mouse anti-FLAG Abs followed by Alexa Fluor 594 goat anti-rabbit Abs (green) and FITC-conjugated goat anti-mouse Abs (red) (E to G), with mouse anti-FLAG and rabbit anti-RASCAL Abs followed by FITC-conjugated goat anti-mouse Abs (green) and Alexa Fluor 594-conjugated goat anti-rabbit Ab (red) (H to J), or with mouse anti-HA and rabbit anti-RASCAL Abs followed by FITC-conjugated goat anti-mouse Abs (green) and Alexa Fluor 594-conjugated goat anti-rabbit Abs (red) (K to M). Bar size, 10 μm.