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. 2010 Apr 21;84(13):6527–6535. doi: 10.1128/JVI.00519-10

FIG. 1.

FIG. 1.

Bulk memory CTL cross-response against pdmH1N1 in healthy individuals. (A) PBMCs from healthy individuals were stimulated with both H1N1 and H3N2 viruses from 2008-2009 seasonal influenza vaccine strains to expand virus-specific bulk CTLs, of which IFN-γ-secreting CD8 T cells (effector [E]) were purified and then cocultured with autologous MDMs (target [T]) infected or not with H1N1, H3N2, and pdmH1N1 viruses at an E/T ratio of 10:1. Data for 12 different individuals are shown. (B) Human influenza virus-specific bulk CTLs were cocultured with pdmH1N1 virus-infected autologous MDMs at the indicated E/T ratios. After 4 h, cell death of MDMs was analyzed by flow cytometry. Data for 12 different individuals are shown. (C) Effector cells were cocultured with MDMs at an E/T ratio of 10:1 in the presence of anti-human CD107a antibody. After 4 h, CD107a expression on CD8 T cells was examined by flow cytometry. Data for 12 different individuals are shown.