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. 2010 Apr 21;84(13):6527–6535. doi: 10.1128/JVI.00519-10

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FIG. 6. — In vivo expansion of M1_58-66 epitope-specific CTLs by seasonal influenza vaccine. PBMCs from healthy HLA-A2-seropositive individuals were isolated before and 1 month after seasonal 2008-2009 influenza vaccination. HLA-A2/M1_58-66 tetramer staining was performed and analyzed by flow cytometry. The numbers shown here indicate the frequency of HLA-A2/M1_58-66-tetramer⁺ cells within the population of CD3⁺ CD8⁺ T cells. Among 20 HLA-A2-positive individuals, only 4 individuals had an increase of HLA-A2/M1_58-66 tetramer-positive CTLs 1 month after vaccination. Results from those four donors are shown.