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. 2010 Apr 28;84(13):6472–6482. doi: 10.1128/JVI.00016-10

FIG. 1.

FIG. 1.

MHV replication is severely restricted and sensitive to type I interferon treatment in oligodendrocytes. (A) Restriction of MHV growth in oligodendrocytes. Oligodendrocyte N20.1 cells or fibroblast 17Cl-1 cells were infected with MHV-A59/GFP at an MOI of 10. Virus titer in culture medium was determined at 24 h p.i. by plaque assay. (B) One-step growth curve of MHV-A59/GFP in N20.1 cells. (C) Sensitivity of MHV replication to IFN treatment. N20.1 cells were treated with either IFN-α or IFN-β at 50, 100, and 200 international units (IU) for 24 h and infected with MHV-A59/GFP at an MOI of 10 in the presence of the respective IFN. At 24 h p.i., virus titer in culture medium was determined by plaque assay. The results are expressed as the mean PFU per ml for three independent experiments. Error bars indicate standard deviations of the means. M, mock treatment.