FIG. 6.

Silencing of the G3BPs has a sustained effect on SINV polyprotein expression and a small enhancing effect on SINV virion production. 293T cells were treated with an irrelevant siRNA (•) or siRNAs targeting G3BP1 and G3BP2 (○) and were infected 2 days later with SINV. (A) Cells seeded in 24-well plates were infected with Toto1101/Luc (MOI of ∼5) and were harvested at the indicated times. The data are the mean luciferase activities relative to the activity of the 0-h irrelevant silenced sample; error bars indicate the standard deviation of triplicate samples. Similar results were obtained in one other independent experiment. (B) Cells seeded in 12-well plates were infected with Toto1101 (MOI of ∼0.01), and the amount of virus released into the medium of duplicate samples was determined by plaque assay titration on BHK-21 cells. Separate wells were utilized for each time point. The data are mean log titers ± the standard errors of the means; error bars are obscured by the symbols. Similar results were obtained in another independent experiment. For both panels A and B, asterisks indicate values significantly different than the irrelevant silenced sample at a given time point (unpaired t test; *, P < 0.05; **, P < 0.01).