FIG. 4.

Rescue of recombinant RF viruses containing in vitro-modified, cDNA-derived rearranged segment 7R or 7RΔ. Recombinant viruses r-RF(7R), r-RF(7R-BstBI), r-RF(7RΔ), and r-RF(7RΔ-EcoRI) are representative clones rescued from MA-104 cell culture. (A) dsRNA profiles of recombinant and control (M1 [7R], M3 [7RΔ], and RF) viruses. Segments 7R and 7RΔ are indicated by arrows. (B) BstBI and EcoRI restriction profiles of RT-PCR products obtained from segments 7 of recombinant and control viruses. +, digested; −, not digested. Numbers indicate the expected sizes of fragments. Mw, 100-bp-ladder molecular size marker.