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The chimeric ZWT Gag construct exhibits L domain dependence when NC-p1 is fused to its C terminus. (A) 293T cells were transfected with a PR-defective HIV-1 provirus expressing WT Pr55gag or with the indicated Gag constructs. VLP pellets and the cell lysates were analyzed by Western blotting with anti-CA serum. (B) Thin sections of 293T cells transiently expressing the ZWT-NCp1p6 or ZWT-NCp1 proviral construct were examined by transmission electron microscopy. Scale bars, 100 nm.
