FIG. 4.
The addition of p1-p6 but not of p6 alone to a chimeric Gag construct confers L domain dependence. (A) Effect of deleting the Tsg101 or the ALIX binding site from a chimeric Gag construct containing p1. 293T cells were transfected with 1.5 μg of WT ZIL-p1p6 or the indicated mutants, along with 0.5 μg of a vector expressing RFP alone or CHMP3-RFP. VLP pellets and the cell lysates were analyzed by Western blotting with anti-CA serum. (B) Effect of deleting the Tsg101 binding site from a chimeric Gag construct lacking p1. 293T cells were transfected with 1.5 μg of WT ZIL-p6 or a ΔPTAPP version.