FIG. 5.
The removal of NC-p1 sequences from authentic HIV-1 Gag alleviates the effect of dominant-negative CHMP3 on particle production. (A) 293T cells were transfected with 1.5 μg of a PR-defective HIV-1 provirus expressing WT Pr55gag or with the indicated NC-p1 deletion mutants, along with a vector expressing RFP alone or CHMP3-RFP. VLP pellets and the cell lysates were analyzed by Western blotting with anti-CA serum. (B) Effect of CHMP3-RFP on the indicated NC zinc finger mutants. Cross-hatched boxes indicate the positions of the zinc fingers in NC, and arrowheads indicate the positions of residues that were substituted. Western blotting was performed with 183-H12-5C anti-HIV CA antibody.