FIG. 2.

(A) Insulin autoantibodies measured by our standard mIAA assay. The horizontal line gives the cutoff (0.01). (B) Standard E-IAA. The horizontal line gives the cutoff (0.01). (C) Competitive CE-IAA. The horizontal line gives the cutoff (0.002). B6, C57BL/6 mice (n = 4–13); Balb, BALB/c mice (n = 20); NZB mice (n = 4–13); NOD (F) and NOD (M), female and male NOD mice (n = 53–57 and n = 10–20, respectively); 2KO(F) and 2KO(M), female and male mice with 2KO (n = 9–17 and n = 6–13, respectively). The samples were randomly selected and not identical in each group (mIAA assay, E-IAA, and CE-IAA).