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. 2010 May 4;285(29):22174–22185. doi: 10.1074/jbc.M109.085464

FIGURE 1.

FIGURE 1.

Orm is induced in the plasma and adipose tissues of obese subjects. A, Northern blot analysis of Orm expression in various mouse tissues. BAT, brown adipose tissue; WAT, white adipose tissue. B, mRNA levels of each Orm isoform in adipocytes and SVCs from epididymal adipose tissues of lean C57BL6 mice. Adiponectin (AdipoQ) was used as an adipocyte-specific marker gene. n = 4. C, Northern blot analysis of Orm expression in the liver and adipose tissue of obese ob/ob and db/db mice. D, Western blot analysis of ORM protein expression in the liver and adipose tissue. As ORMs are heavily glycosylated proteins (about 45% of their total mass is composed of carbohydrates), Western blot analysis of ORM with tissue samples accompanies both its glycosylated as well unglycosylated forms, as illustrated. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. E, ORM protein level in the plasma of lean and obese mice. n = 4 or 5. F, correlation of plasma ORM level with body mass index (BMI). Serum ORM level was measured by enzyme-linked immunosorbent assay and plotted in accordance with body mass index. R = 0.3, p = 0.021.