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. 2010 May 11;285(29):22211–22220. doi: 10.1074/jbc.M110.119982

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Circulating oxidatively truncated phospholipids increase during chronic ethanol ingestion. A, sn-2 glutaroyl choline phospholipids. PG-PC, palmitoyl glutaroyl phosphatidylcholine; G-LPAF, glutaroyl lysoPAF (LPAF, 1-O-hexadecyl-sn-glycero-3-phosphocholine). B, sn-2 azelaoyl choline phospholipids. AZ-PC, azelaoyl phosphatidylcholine; AZ-LPAF, azelaoyl lysoPAF. C, platelet-activating factor. D, plasma PAF acetylhydrolase (PAFAH) activity. Plasma was from rats (n = 5) maintained on the Lieber-DeCarli ethanol diet for 4 weeks or their pair-fed controls. PAF and the truncated phospholipids were quantified by LC/MS/MS using [²H₄]PAF as the internal standard. PAF acetylhydrolase activity was quantified using [acetyl-³H-]PAF. Alkyl phospholipids contain a lysoPAF, rather than a lysophosphatidylcholine, backbone. *, p < 0.05 comparing ethanol-fed to pair control.