FIGURE 4.

Chronic ethanol feeding induces hepatic oxidative stress and hepatocyte damage. A, 4-hydroxynonenol immunohistochemistry. Sections of livers from rats treated for the stated time and conditions were sectioned and stained as described under “Experimental Procedures.” W, weeks; E, ethanol; P, pair-fed. B, circulating alanine transaminase levels. ALT was assessed in blood drawn from rats at the stated times and conditions by the Cleveland Clinic medical laboratory. *, p < 0.05 comparing ethanol-fed to pair-fed controls, &, p < 0.05 comparing animals fed ethanol for a different period.