FIGURE 2.

The ratio between IαI and TSG-6 during incubation determines the variant of bikunin complexes formed. Shown is a Coomassie Blue-stained reduced SDS-PAGE gel with IαI (lane 1) and TSG-6 (lane 2) as controls. A fixed concentration of IαI was incubated with increasing amounts of TSG-6, 1:1 (lane 3), 1:2 (lane 4), 1:3 (lane 5), 1:5 (lane 6), and 1:7 (lane 7) (molar ratios). The titration demonstrates that the outcome of IαI/TSG-6 interaction is dependent on the IαI:TSG-6 ratio. This is most apparent by observing the decreasing amount of HMW IαI formed when the amount of TSG-6 is increasing.