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. 2010 May 12;285(29):21988–21993. doi: 10.1074/jbc.M109.041046

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — TSG-6/HC2 transfers HCs from HA to HA. Shown is a Coomassie Blue-stained reduced SDS-PAGE gel showing samples of IαI (lane 1), IαI incubated with TSG-6 (lane 2), IαI and TSG-6 incubated with HA20 and subsequently with an excess of HA12 (lane 3), IαI and TSG-6 incubated with HA20 (lane 4), and IαI dissociated by NaOH (lane 5). Close inspection of the gel (see inset) revealed that the HC·HA molecules, generated after incubation with an excess HA12, migrated faster than the HC·HA molecules generated after incubation with HA20 only, as indicated by the arrow (see inset). The bands of interest are magnified to illustrate the band shift between HC·HA12 and HC·HA20. The experiment shows that the HCs in HC·HA20 are transferred to HA12, which demonstrates that the HC·HA complexes are HC donors in the TSG-6/HC2-mediated HC transfer reaction.