TABLE 1.

Comparison of wild-type and mutant FlK

Enzyme reactions were carried out using FAcCoA as the substrate. Errors quoted represent the S.E. of curve fitting (Lineweaver-Burk plot). Each data point is the average of four (WtFlK), three (T42SFlK), or two E50AFlK measurements.

Protein	Soluble protein	kcata	Km	kcat/Kmb	FAcCoA concentration above which substrate inhibition was observed
	mg/liter	s−1	μm	mm−1s−1	μm
WtFlK	∼10–12	0.044 ± 0.001	30 ± 1.3	1.47 ± 0.10
T42SFlK	∼10–12	0.409 ± 0.159	15 ± 1.2	27.3 ± 12.8	12
T42AFlK	∼2
E50AFlK	∼2	0.111 ± 0.041	206 ± 67	0.54 ± 0.37	19

^a The units are number of CoA released/s/enzyme molecule.

^b The error values given for k_cat/K_m are calculated from the sum of the relative errors on values in the numerator and denominator.