FIGURE 2.

MLK-dependent induction of proapoptotic markers in SICC. A, SICC for 24 h with unstimulated (row i) and stimulated splenocytes pretreated with DMSO (row ii) or with CEP11004 (row iii) were used to detect MLK3, TUNEL, TRB3, and conformationally altered BAX using monoclonal antibody clone 6A7 as described (columns 1–4, respectively). B, quantification of TUNEL (*, p < 0.001 versus unstimulated media; **, p < 0.001 versus stimulated medium as tested by ANOVA followed by Bonferroni post hoc test) represents means ± S.E. (error bars) of three independent experiments. C, pancreatic sections from prediabetic and diabetic NOD mice were immunostained for TRB3 (panels i and ii) or BAX-6A7 (panels iii and iv). Bar, 20 μm.