FIGURE 8.

LPCAT3 knockdown cells are susceptible to palmitic acid exposure. HeLa cells were transfected with the indicated siRNA. At 60 h after transfection cells were further incubated for 12 h in media supplemented with 16:0 (100 μm) and then harvested. A and B, expressions of CHOP (A) and GRP78 (B) mRNAs were detected by quantitative real-time PCR. The expression level of each gene was normalized to the GAPDH gene and is represented as -fold induction over siControl treated with ethanol. C, semiquantitative reverse transcription-PCR analysis of XBP1 spliced and unspliced mRNA is shown. The positions of the unspliced form (u) and spliced form (s) are indicated. Thapsigargin-treated cells (Tg) were used as a positive control. The asterisks indicate significant differences compared with siControl-transfected cells, which were treated with ethanol (p < 0.01).