FIGURE 2.

Clock mutation damps circadian variation of GYS2 protein in both fed and fasted mice. Wild-type (WT) and Clock mutant mice were maintained under a 12 h of light/12 h of dark cycle (lights on at 0:00 and lights off at 12:00) and fed (left panel) or fasted (right panel) overnight. Protein was extracted from the livers of these mice, and then the GYS2 protein level was determined by Western blotting using anti-GYS2 antiserum. Open and closed symbols, wild-type and Clock mutant mice, respectively. The maximal value for wild-type mice is expressed as 100%. The values are the means ± S.E. (n = 3). The significant differences compared with the values from wild-type mice at each time point are indicated. *, p < 0.05; †, p < 0.01. The p values were calculated using Student's t test.