FIGURE 5.

Recruitment of ERβ to class I, II, and II genes in U2OS cells. U2OS-ERβ cells were treated with or without doxycycline for 18 h, followed by vehicle or 10 nm E₂ treatment for 1 h. Following treatments, ChIP for ERβ was performed. Recruitment of ERβ to the class I genes, MSMB (A) and TMOD1 (B), class II genes CECR6 (C) and DHRS9 (D), or class III genes CST5 (E) and HAVCR2 (F) were measured by real-time PCR (n = 3). Each data point is the average of triplicate determinations ± SEM. Asterisk denotes p value ≤0.05.