TABLE 3.

Genome-wide distribution of ERβ binding sites

ERβ-expressing U2OS cells were treated without (unliganded) and with (liganded) 10 nm E₂ for 1 h. Peak regions were associated with the HG18 RefGene table downloaded from the University of California, Santa Cruz genome browser. Peaks within 5 kb upstream and 2.5 kb downstream of a RefSeq gene TSS were annotated as being in the promoter region. Peaks within 2.5 kb downstream of a TSS and 2.5 kb upstream of 3′ end of the gene were annotated as intragenic. Peaks within 2.5 kb upstream of 3′ end and 5 kb downstream of 3′ end were annotated as the 3′ end. Peaks within 50 kb of a gene but excluded from the above three categories were annotated as within 50 kb. Peaks falling outside of 50 kb window of any gene were labeled as others and considered as peaks involved in long range interactions.

Distribution	Unliganded ERβ	Liganded ERβ
	%	%
Promoter	13	12
Intragenic	25	25
3′ end	2	2
Within 50 kb	36	38
Others	24	23