Distribution of thresholded Mander's coefficients (tM) of colocalization of EqtII-GFP or EqtII Y113A-GFP and Bodipy-TR-ceramide. The MDCK cells were also analyzed in the presence of 25 μg/ml of D609, while the NIH 3T3 fibroblasts (FB) were also analyzed in the presence of 50 μg/ml BFA. 0 means no colocalization, 1 is perfect colocalization. The average (shown by cross) ± S.D. is also presented. The differences between groups were tested using t test. *, p < 0.05; **, p < 0.01; n.s., not significant.