FIGURE 3.

Transgenic VP16ERRγ mice have increased muscle mitochondrial function. A, representative images of succinate dehydrogenase (SDH) staining in tibialis muscle in littermate control (WT) (left panel) and VP16ERRγ transgenic (TG) (right panel) mice; B, staining intensity (left panel) and total staining area (right panel) of the muscle cross-sections (n = 5–6 mice per group). Enzymatic activities for citrate synthase (CS) (C) and aconitase (D) were determined in gastrocnemius muscle (n = 6–8 mice per group). Gene expression for MHCs (E) and metabolic and mitochondrial genes (F) (n = 6–8 mice per group) are shown. Data are presented as mean ± S.E. Statistical significance is indicated as follows: *, p < 0.05; **, p < 0.01; ***, p < 0.005. G, heat maps denoting individual genes and pathways that were altered in gastrocnemius muscle of TG mice relative to littermate control mice. The color scheme representing the fold change is shown.