TABLE 1.
Time constants of xASIC1.1 mutants
Whole-cell currents evoked by pH 7.1 in oocytes expressing xASIC1.1 wild type, single mutant, and triple mutant. The values of the activation τa and desensitization τd time constants were obtained by fitting the time course of the macroscopic currents to Equation 2 (see “Experimental Procedures”). ND, not determined.
| Current (mean ± S.D.) | No. of cells | τa (mean ± S.D.) | τd (mean ± S.D.) | |
|---|---|---|---|---|
| μA/cell | n | ms | ms | |
| Wild type Xenopus | 3 ± 0.7 | 24 | 40 ± 8 | 180 ± 30 |
| S83Q84L85 | 10 ± 4 | 26 | 230 ± 28 | 3100 ± 350 |
| P83S | 4 ± 0.8 | 9 | 52 ± 3 | 260 ± 40 |
| K84Q | 1.8 ± 0.4 | 8 | 51 ± 4 | 270 ± 30 |
| M85L | 9 ± 0.11 | 25 | 220 ± 20 | 2920 ± 180 |
| M85T | 0.8 ± 0.4 | 11 | 12 ± 2 | 70 ± 13 |
| M85F | 1.1 ± 0.2 | 5 | 11 ± 3 | 65 ± 20 |
| M85C | 2.8 + 0.4 | 6 | 42 ± 82 | 222 ± 40 |
| M85A | 1.5 ± 0.3 | 5 | 40 ± 3 | 190 ± 30 |
| M85R | 0.2 ± 0.1 | 12 | ND | ND |