TABLE 1.

Mapping Plk1 phosphorylation site(s) of survivin

Survivin construct	Phosphorylation	Expression	Solubility
1–142 (full-length)	++	Good	Soluble
1–115	++	Good	Soluble
1–90	++	Good	Soluble
90–142	−	Good	Soluble
98–142	−	Good	Soluble
30–142	−	Good	Soluble
S20A	−	Good	Soluble
T21A	−	Good	Mostly insoluble
S20A/T21A	−	Good	Mostly insoluble
T48A	++	Good	Soluble
T97A	++	Good	Soluble