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. 2010 May 14;285(29):22360–22369. doi: 10.1074/jbc.M109.059949

FIGURE 3.

FIGURE 3.

Inhibition of Nur77 transactivation on the promoter of steroidogenic enzyme genes by ARR19. A, upper panel, MA-10 cells were cultured on 24-well plates and transfected with 150 ng of NurRE-Luc reporter, 50 ng of pcDNA3-Nur77, 100 ng of pCMV-β-galactosidase, and increasing amounts (50, 100, and 200 ng) of pcDNA-HA-ARR19. After 36 h, the cells were harvested, and luciferase activities were determined and normalized with β-galactosidase activity for the transfection efficiency. Lower panel, expression levels of Nur77 and ARR19 protein were ascertained by Western blot analyses of 50 μg of cellular extracts from the transient transfections. B, upper panel, MA-10 cells were transfected with 150 ng of SF-1RE-Luc reporter, 50 ng of pcDNA3-SF-1, 100 ng of pCMV-β-galactosidase, and increasing amounts (50, 100, and 200 ng) of pcDNA-HA-ARR19. Luciferase activities were determined and normalized as in A. Lower panel, expression levels of SF-1 and ARR19 protein were ascertained by Western blot analyses as described in A. C–E, MA-10 cells were transiently transfected with 150 ng of the indicated reporter, StAR-Luc (C), P450c17-Luc (D), and 3β-HSD-Luc (E), together with pcDNA-Nur77 and an increasing amount of ARR19 expression vector (50, 100, and 200 ng). Luciferase activities were determined and normalized as described for A.