TABLE 5.
PAF reversed the effect of glucosamine-PI on PrPC distribution
The amount of PrPC in DRMs (lipid rafts) or non-raft (detergent-soluble fractions) of membranes isolated from GT1 cells treated for 24 h with combinations of 1 μm glucosamine-PI and 1 μm PAF, 1 μm C-PAF, or 1 μm lyso-PAF is as shown. Values are the mean average amount of PrPC (ng/106 cells) ± S.D., n = 12. Also shown is the amount of PrPC expressed at the surface of GT1 cells incubated for 24 h with combinations of 1 μm glucosamine-PI and 1 μm PAF, 1 μm C-PAF, or 1 μm lyso-PAF as shown. Values are the mean average amount of cell surface PrPC (ng/106 cells) ± S.D., n = 12.
| Treatment | PrPC ng/106 cells |
||
|---|---|---|---|
| Lipid raft | Non-raft | Cell surface | |
| Vehicle-treated | 26.7 ± 2.1 | 3.6 ± 1.4 | 5.9 ± 0.8 |
| 1 μm glucosamine-PI | 4.8 ± 0.9a | 23.9 ± 3.8 | 0.5 ± 0.4a |
| 1 μm glucosamine-PI + 1 μm PAF | 28.4 ± 3.7 | 4.5 ± 1.1 | 5.4 ± 1.1 |
| 1 μm glucosamine-PI + 1 μm C-PAF | 30 ± 3.6 | 4.2 ± 1.6 | 6.3 ± 1.8 |
| 1 μm glucosamine-PI ± 1 μm lyso-PAF | 3.5 ± 1.7a | 22.2 ± 5.4 | 1.2 ± 0.6a |
a Amount of PrPC is significantly less than that of vehicle-treated cells.